|
| Status |
Public on Jul 23, 2013 |
| Title |
CD4+ T cells, post treatment, biological replicate 4 |
| Sample type |
RNA |
| |
|
| Source name |
CD4+ T cells, post treatment
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: sarcoidosis
cell type: CD4+ T cells
|
| Treatment protocol |
Participants received the oral CLEAR regimen: Levofloxacin 750 mg on day 1, then 500 mg daily, Ethambutol 1200 mg daily, Azithromycin 500 mg on day 1, then 250 mg daily, and Rifampin 300 mg daily, or a comparative placebo regimen, for a total of 8 weeks, with a 180 day follow-up.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using miRNeasy mini kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Cy3 labeled RNA was prepared according to the standard Agilent protocol from 50ng total RNA
|
| |
|
| Hybridization protocol |
Labeled RNA was hybridized for 17 hr at 65C on Agilent 8X60K microRNA array. Arrays were washed according to the manufacturer's protocol.
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 8x60K array slides
|
| Description |
C3-4_310
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 10.7.3.1 (Agilent) using default parameters.
|
| |
|
| Submission date |
Jul 24, 2012 |
| Last update date |
Jul 23, 2013 |
| Contact name |
Kusum Vijay Pandit |
| E-mail(s) |
panditkusum@yahoo.com
|
| Phone |
412-624-9046
|
| Organization name |
University of Pittsburgh
|
| Department |
Medicine-PACCM
|
| Street address |
200, Lothrop street, W1200-25A Biomedical Science Tower
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
| |
|
| Platform ID |
GPL14550 |
| Series (1) |
| GSE39606 |
Gene expression data in CD4+ and CD8+ cells in sarcoidosis patients following antimycobacterial therapy |
|
