|
Status |
Public on Aug 11, 2012 |
Title |
Bone marrow mesenchymal stem cells from aborted fetus,replicate 2 |
Sample type |
RNA |
|
|
Source name |
Bone marrow mesenchymal stem cells from aborted fetus 2
|
Organism |
Homo sapiens |
Characteristics |
tissue: bone marrow cell type: mesenchymal stem cells subject status: aborted fetus
|
Growth protocol |
Bone marrow mononuclear cells (BMMNC) of 7 psoriatic patients, 4 healthy volunteers and 3 aborted fetuses were isolated and the BMMSCs were cultured using the adherent method.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from BMMNC was extracted using Trizol reagent (Invitrogen, Gaithersburg, MD) according to the manufacturer’s instructions.
|
Label |
Cy5
|
Label protocol |
RNA was amplified and labeled with Cy5 using Agilent’s Low Input Quick Amp WT Labeling Kit according to the manufacturer’s instruction
|
|
|
Hybridization protocol |
According to the instructions of Agilent Gene Expression Hybridization Kit.
|
Scan protocol |
Scanned on an Agilent G2505C scanner.
|
Description |
aborted fetus US10283824_252800411426_S01_GE1_107_Sep09_1_3
|
Data processing |
Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1).Agilent GeneSpring GX 11.5 was used for background subtraction and LOWESS normalization.
|
|
|
Submission date |
Aug 10, 2012 |
Last update date |
Aug 11, 2012 |
Contact name |
wang liang |
E-mail(s) |
wangliangkexue@163.com
|
Organization name |
BioChainBJ
|
Street address |
7A North Yongchang Road BDA
|
City |
Beijing |
ZIP/Postal code |
100176 |
Country |
China |
|
|
Platform ID |
GPL14550 |
Series (1) |
GSE40033 |
The gene expression pattern of bone marrow mesenchymal stem cells from patients with psoriasis |
|