|
| Status |
Public on Aug 11, 2012 |
| Title |
Bone marrow mesenchymal stem cells from psoriasis patient,replicate 6 |
| Sample type |
RNA |
| |
|
| Source name |
Bone marrow mesenchymal stem cells from psoriasis patient 6
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: bone marrow
cell type: mesenchymal stem cells
subject status: psoriasis patient
|
| Growth protocol |
Bone marrow mononuclear cells (BMMNC) of 7 psoriatic patients, 4 healthy volunteers and 3 aborted fetuses were isolated and the BMMSCs were cultured using the adherent method.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from BMMNC was extracted using Trizol reagent (Invitrogen, Gaithersburg, MD) according to the manufacturer’s instructions.
|
| Label |
Cy5
|
| Label protocol |
RNA was amplified and labeled with Cy5 using Agilent’s Low Input Quick Amp WT Labeling Kit according to the manufacturer’s instruction
|
| |
|
| Hybridization protocol |
According to the instructions of Agilent Gene Expression Hybridization Kit.
|
| Scan protocol |
Scanned on an Agilent G2505C scanner.
|
| Description |
psoriasis patient
US10283824_252800411426_S01_GE1_107_Sep09_2_3
|
| Data processing |
Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1).Agilent GeneSpring GX 11.5 was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Aug 10, 2012 |
| Last update date |
Aug 11, 2012 |
| Contact name |
wang liang |
| E-mail(s) |
wangliangkexue@163.com
|
| Organization name |
BioChainBJ
|
| Street address |
7A North Yongchang Road BDA
|
| City |
Beijing |
| ZIP/Postal code |
100176 |
| Country |
China |
| |
|
| Platform ID |
GPL14550 |
| Series (1) |
| GSE40033 |
The gene expression pattern of bone marrow mesenchymal stem cells from patients with psoriasis |
|
