GEO DataSets

(Submitter supplied) In this study, we established a protocol based on the HEV genotype 3 p6 (Kernow C-1) and the human hepatoma cell lines HepG2 and HepG2/C3A with different media conditions to produce HEV cell culture-derived particles (HEVcc) with viral titers between 10e5 to 10e6 FFU/mL. Viral titers could be further enhanced by an HEV variant harboring a mutation in the RNA-dependent RNA polymerase. These HEVcc particles were infectious in primary human hepatocytes. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

24 Samples

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(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Sus scrofa; Homo sapiens

Type:

Expression profiling by RT-PCR

Platforms:

GPL33783 GPL33782 GPL33776

52 Samples

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(Submitter supplied) We aimed to identify interferon (IFN)-regulated genes that are differentially expressed during HEV infection in swine liver tissues.

Organism:

Sus scrofa

Type:

Expression profiling by RT-PCR

Platform:

GPL33783

8 Samples

Download data: TXT

(Submitter supplied) We aimed to identify interferon (IFN)-regulated genes that are differentially expressed during chronic HEV infection in human hepatocytes, the main site of HEV replication, using HepaRG cells.

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL33782

12 Samples

Download data: TXT

(Submitter supplied) We aimed to identify interferon (IFN)-regulated genes that are differentially expressed during chronic HEV infection in human hepatocytes, the main site of HEV replication, using HepaRG cells.

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL33776

12 Samples

Download data: TXT

(Submitter supplied) We aimed to identify interferon (IFN)-regulated genes that are differentially expressed during chronic HEV infection in human hepatocytes, the main site of HEV replication, using HepaRG cells. We have performed a preliminary screen using whole-cell RNA extracts prepared from HepaRG mock-infected or infected cells to determine whether HEV was able to trigger an IFN response. Different multiplicities of infection (MOI) (10 and 100 genome equivalent (GE)/cell) and time points (D+7, D+14, D+26, D+40, D+72 and D+100) were analyzed using the PCR array. more...

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL33776

20 Samples

Download data: TXT

(Submitter supplied) We used whole genome expression profiling to analyze the effects of Hepatitis E Virus (HEV) on the mRNA transcriptome of A549/D3 cells - particularly to identify differentially expressed genes which are associated with cholesterol metabolism.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17077

12 Samples

Download data: TXT

(Submitter supplied) Human stem cell-derived hepatocyte-like cells (HLCs) offer an attractive platform to study liver disease and to test drugs. However, despite their advantages over other hepatocyte culture systems, HLCs lack several in vivo characteristics including cell polarity, which is critical for proper hepatocyte biology. We report a stem cell-based differentiation protocol that uses transwell filters to generate columnar polarized HLCs with clearly defined basolateral and apical membranes separated by tight junctions. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

4 Samples

Download data: CSV