|
| Status |
Public on May 07, 2019 |
| Title |
Ultra-High Throughput Single Cell Analysis of Proteins and RNAs by Split-pool Synthesis |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Other
Expression profiling by high throughput sequencing
|
| Summary |
We report a method enabling simultaneous, ultra-high throughput single-cell-barcoding, of millions of cells for targeted single cell analysis of proteins and RNAs. This method termed Quantum Barcoding (QBC) circumvents the need to isolate single cells by building cell-specific oligo barcodes dynamically within each cell. With minimal instrumentation (four 96-well plates and a multichannel pipette) cell-specific codes are added to each tagged molecule within cells. This is accomplished through sequential rounds of the well-established process of classic “split-pool synthesis”. We demonstrate the utility of this currently research use only technology in multiple model systems.
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| |
|
| Overall design |
Analysis of various cell types by the QBC single-cell analysis technology
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| |
|
| Contributor(s) |
O’Huallachain M, Paladugu S |
| Citation(s) |
32382044 |
| Submission date |
May 06, 2019 |
| Last update date |
May 14, 2020 |
| Contact name |
Maeve Ohuallachain |
| E-mail(s) |
maeve.ohuallachain@roche.com
|
| Organization name |
ROCHE SEQUENCING SOLUTIONS INC
|
| Street address |
4300 Hacienda Drive
|
| City |
Pleasanton |
| State/province |
CALIFORNIA |
| ZIP/Postal code |
94588 |
| Country |
USA |
| |
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| Platforms (6)
|
| GPL15103 |
Illumina HiSeq 1000 (Mus musculus) |
| GPL15433 |
Illumina HiSeq 1000 (Homo sapiens) |
| GPL15520 |
Illumina MiSeq (Homo sapiens) |
| GPL16417 |
Illumina MiSeq (Mus musculus) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL19415 |
Illumina NextSeq 500 (Homo sapiens; Mus musculus) |
|
| Samples (24)
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|
| Relations |
| BioProject |
PRJNA541327 |
| SRA |
SRP195639 |
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