|
| Status |
Public on Aug 29, 2012 |
| Title |
96 mRRBS libraries: Gel-free multiplexed reduced representation bisulfite sequencing for large-scale DNA |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
Sequencing based approaches have led to new insights about DNA methylation. While many different techniques for genome-scale mapping of DNA methylation have been employed, throughput has been a key limitation for most. To further facilitate the mapping of DNA methylation, we describe a protocol for gel-free multiplexed reduced representation bisulfite sequencing (mRRBS) that reduces the workload dramatically and enables processing of 96 or more samples per week. mRRBS achieves similar CpG coverage as the original RRBS protocol, while the higher throughput and lower cost make it better suited for large-scale DNA methylation mapping studies including cohorts of cancer samples.
|
| |
|
| Overall design |
Libraries of 96 human samples
|
| |
|
| Contributor(s) |
Patrick B, Kendell C, Andreas G, Alexander M |
| Citation(s) |
23034176 |
| Submission date |
Aug 28, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Kendell Clement |
| Organization name |
Harvard University
|
| Lab |
Meissner Lab
|
| Street address |
7 Divinity Ave.
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (96)
|
|
| Relations |
| BioProject |
PRJNA173930 |
| SRA |
SRP015245 |
Less...
More...