|
| Status |
Public on Jul 10, 2017 |
| Title |
Experimental validation of candidate regulators of KRAS transcriptional components. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We sequenced mRNA from HCC44 and YAPC cell lines stably expressing Cas9, and infecting with indicated gRNAs against regulators of KRAS transcriptional components.
|
| |
|
| Overall design |
KRAS mutant cell lines HCC44 and YAPC stably expressing with Cas9 were infected with three independent gRNAs targeting indicated genes or controls (GFP, RFP, random gRNAs) and harvested 8 days after infection.
|
| |
|
| Contributor(s) |
Kim JW, Mesirov J, Hahn WC, Tamayo P |
| Citation(s) |
28837809 |
| Submission date |
Jul 21, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Jong Wook Kim |
| E-mail(s) |
jongw@broadinstitute.org
|
| Organization name |
Broad Institute
|
| Department |
Cancer Program
|
| Street address |
45 Main Street
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (21)
|
|
| Relations |
| BioProject |
PRJNA330865 |
| SRA |
SRP079257 |
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