|
| Status |
Public on Jun 30, 2015 |
| Title |
Nasopharyngeal Carcinoma tissue 5 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
human biopsy of nasopharyngeal carcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
biopsy: human biopsy of nasopharyngeal carcinoma
diagnosis: undifferentiated Nasopharyngeal Carcinoma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Hy5
|
| Label protocol |
6 µg of total RNA were labeled with labeling protocal of miRCURY LNA microRNA Array, Power labeling Kit
|
| |
|
| Channel 2 |
| Source name |
Total RNA from Human lung fibroblasts cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HLF
passage: 6
cell type: HLF drived from Human lung fibroblasts
|
| Growth protocol |
HLF cells were cultured in RPMI-1640 with 10% Fetal Cow Serum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Hy3
|
| Label protocol |
6 µg of total RNA were labeled with labeling protocal of miRCURY LNA microRNA Array, Power labeling Kit
|
| |
|
| |
| Hybridization protocol |
Oligoarray control targets and hybridization buffer were added, and samples were applied to microarrays enclosed in LF-I molecular hybridization chambers(Scientz, China). After hybridization, slides were washed sequential
|
| Scan protocol |
Scanned on an CapitalBio LuxScan 10K scanner.
Images were quantified using Genepix pro Software (version 6.0).
|
| Description |
Biological replicate 5 of 20. Nasopharyngeal Carcinoma tissue 5
NPC5
|
| Data processing |
LOWESS normalized, background subtracted data obtained from log2 of processed Red signal/processed Green signal. Madb and BRB ArrayTools were used.
|
| |
|
| Submission date |
Dec 19, 2012 |
| Last update date |
Jun 30, 2015 |
| Contact name |
Xin Li |
| E-mail(s) |
xinli268@gmail.com
|
| Phone |
86 20 62789438
|
| Organization name |
Southern medical university
|
| Department |
Cancer research institute
|
| Street address |
Southern medical university
|
| City |
Guangzhou |
| State/province |
Province |
| ZIP/Postal code |
510515 |
| Country |
China |
| |
|
| Platform ID |
GPL16414 |
| Series (2) |
| GSE42945 |
The novel functional effects of exogenous EBV-miR-BARTs on NPC progression |
| GSE43039 |
microRNA expression profiling of human nasopharyngeal Carcinoma tissues and Chronic Nasopharyngitis specimens |
|
