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Status |
Public on Jul 03, 2017 |
Title |
7a: PC9+TSA (50nM ,5hours) RNA-seq |
Sample type |
SRA |
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Source name |
NSCLC
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Organism |
Homo sapiens |
Characteristics |
cell line: PC9 treatment: PC9+TSA (50nM ,5hours)
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Treatment protocol |
PC9 cells were treated with 50nM TSA for five hours. No media change.
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Growth protocol |
PC9 cells were cultured at 37 °C with 5% CO2 in RPMI-1640 (Invitrogen) supplemented with 10% FBS (HyClone).
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Extracted molecule |
polyA RNA |
Extraction protocol |
Total RNA was isolated from above samples using Qiagen RNAeasy extraction kit. RNA was treated with DNAse. ERCC spike-in control was added based on cell number according to manufacturers recommendations. Libraries were generated from poly-A-selected RNA
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
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Data processing |
The expression quantification was done using the Salmon method (Patro, R., Duggal, G., Love, M. I., Irizarry, R. A. & Kingsford, C. Nat Meth (2017))
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Submission date |
Jul 03, 2017 |
Last update date |
May 15, 2019 |
Contact name |
Suchit Jhunjhunwala |
E-mail(s) |
suchitj@gene.com
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Organization name |
Genentech
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Street address |
1 DNA Way, MS-444A
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City |
South San Francisco |
State/province |
CA |
ZIP/Postal code |
94080 |
Country |
USA |
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Platform ID |
GPL18573 |
Series (2) |
GSE74180 |
Repression of stress-induced LINE-1 expression protects cancer cell populations from lethal drug-exposures |
GSE100751 |
Repression of stress-induced LINE-1 expression protects cancer cell populations from lethal drug-exposures [RNA-Seq] |
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Relations |
BioSample |
SAMN07312200 |
SRA |
SRX2980157 |
Supplementary data files not provided |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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