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Sample GSM2977010 Query DataSets for GSM2977010
Status Public on Feb 02, 2018
Title PRMT1_ChIPseq_Ctrl_rbmab
Sample type SRA
 
Source name human keratinoctyes expressing HA-PRMT1
Organism Homo sapiens
Characteristics cell type: primary human neonatal keratinocytes
antibody: HA (cell signaling C29F4)
Treatment protocol HA-PRMT1 were expressed using pBABE retroviral vector at near endogenous level. D4476 were added to 60uM final concentration for 48 hours before crosslinking
Growth protocol neonatal keratinocyte were isolated from discarded foreskin, cultured in KSF and 154 keratinocyte medium
Extracted molecule genomic DNA
Extraction protocol For ChIP-seq, HA-PRMT1 expressing primary keratinocytes were crosslinked with 1% formaldehyde and DSG, sonicated, and immunoprecipiated with antibodies recognizing the HA tag.
For ChIP-seq, NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina was used for library construction.
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina NextSeq 500
 
Description ChIP-seq in undifferentiated keratinocytes
Data processing Reads were mapped to UCSC hg19 using bowtie,
ChIPseq peaks were called using MACS14.
Genome_build: hg19
Supplementary_files_format_and_content: Peak files are in standard bed/whole bed format
 
Submission date Feb 02, 2018
Last update date Oct 11, 2022
Contact name Douglas Porter
Organization name Stanford
Department Dermatology
Lab Khavari
Street address 269 Campus Drive
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platform ID GPL18573
Series (2)
GSE81942 PRMT1 and CSNK1a1 control epidermal progenitor maintenance
GSE110050 PRMT1 and CSNK1a1 control epidermal progenitor maintanence (PRMT1 ChIP-seq data set)
Relations
BioSample SAMN08456675
SRA SRX3642947

Supplementary file Size Download File type/resource
GSM2977010_ctrl_rbmab_peaks.bed.gz 260.0 Kb (ftp)(http) BED
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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