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Sample GSM7567229 Query DataSets for GSM7567229
Status Public on Jul 11, 2023
Title MDCK Single culture_1
Sample type RNA
 
Source name MDCK-pTR GFP-RasV12 cells culture alone
Organism Canis lupus familiaris
Characteristics culture condition: single culture
Treatment protocol MDCK-pTR GFP-RasV12 cells or a 10:1 mix culture of MDCK and MDCK-pTR GFP-RasV12 cells were cultured at a density of 1.2×107 cells on collagen-coated 10 cm dishes (CORNING). After incubation with tetracycline for 24 h at 37 ºC in a humidified incubator with 5% CO2, GFP-positive RasV12 cells was separated with an analytical flow cytometer.
Growth protocol MDCK and MDCK-pTR GFP-RasV12 cells were cultured in Dulbecco's Modified Eagle Medium, High Glucose supplemented with 10% Fetal Bovine Serum and penicillin/Streptomycin at 37 ºC in a humidified incubator with 5% CO2.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from MDCK-pTR GFP-RasV12 mCherry-β-catenin Δ131 cells using ISOGEN II (NIPPON GENE Co., Ltd).
Label Cy3
Label protocol For cRNA amplification and labeling, Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies) were used following the manufacter’s instructions.
 
Hybridization protocol 1.5 ug of Cy3-labelled cRNA was fragmented at 60°C for 30 minutes in a Agilent fragmentation buffer and was hybridized at 65°C for 17 hr using Gene Expression Hybridization Kit.
Scan protocol Microarray slides were scanned using an Agilent SureScan (G4900DA).
Description Gene expression after 24h of tetracycline treatment
Data processing The scanned images were analyzed with Feature Extraction Software (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
 
Submission date Jul 06, 2023
Last update date Jul 11, 2023
Contact name Shunsuke Kon
E-mail(s) kon44@rs.tus.ac.jp
Phone +81-4-7121-4053
Organization name Tokyo University of Science
Department Research Institute for Biomedical Sciences
Lab Division of Cancer Biology
Street address 2669 Yamazaki
City Noda-shi
State/province Chiba
ZIP/Postal code 278-0022
Country Japan
 
Platform ID GPL15379
Series (1)
GSE236658 Gene expression signatures of MDCK-pTR GFP-RasV12 cells cultured alone or mixed with MDCK cells

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
A_11_P085196 -2.867
A_11_P187033 -3.213
A_11_P166448 3.837
A_11_P163658 0.607
A_11_P198798 0.582
A_11_P0000024888 1.063
A_11_P0000011420 0.351
A_11_P177878 -0.840
A_11_P000008463 -3.151
A_11_P168333 -0.891
A_11_P0000020410 -3.339
A_11_P091711 -0.090
A_11_P130416 -0.393
A_11_P167898 3.503
A_11_P213888 -4.975
A_11_P052641 -5.801
A_11_P0000040688 -5.041
A_11_P0000020760 -0.018
A_11_P0000021178 2.341
A_11_P0000015207 -5.367

Total number of rows: 26529

Table truncated, full table size 558 Kbytes.




Supplementary file Size Download File type/resource
GSM7567229_alone1.txt.gz 8.7 Mb (ftp)(http) TXT
Processed data included within Sample table

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