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| Status |
Public on Jul 11, 2023 |
| Title |
Gene expression signatures of MDCK-pTR GFP-RasV12 cells cultured alone or mixed with MDCK cells |
| Organism |
Canis lupus familiaris |
| Experiment type |
Expression profiling by array
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| Summary |
Newly emerging transformed cells carrying genetic defects, including the active Ras mutant, are apicall extruded when surrounded by normal epithelial cells. This biological phenomenon is termed as cell competition. It remains elusive which factors prime RasV12-transformed cells to be expelled via cell competition. Here, We performe microarray analysis to search for molecules whose expression is changed in RasV12 cells surrounded by normal cells.
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| Overall design |
1.2×10^7 of 10:1 mix culture of MDCK and MDCK-pTR GFP-RasV12 cells or a single culture of MDCK-pTR GFP-RasV12 cells were cultured in collagen type I-coated dishes. After incubation with tetracycline for 24 h, GFP-positive cells were collected by an analytical flow cytometer, and total RNA was extracted from the isolated cells.
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| Contributor(s) |
Ishibashi K, Kon S |
| Citation(s) |
37923722 |
| Submission date |
Jul 06, 2023 |
| Last update date |
Dec 01, 2023 |
| Contact name |
Shunsuke Kon |
| E-mail(s) |
kon44@rs.tus.ac.jp
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| Phone |
+81-4-7121-4053
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| Organization name |
Tokyo University of Science
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| Department |
Research Institute for Biomedical Sciences
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| Lab |
Division of Cancer Biology
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| Street address |
2669 Yamazaki
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| City |
Noda-shi |
| State/province |
Chiba |
| ZIP/Postal code |
278-0022 |
| Country |
Japan |
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| Platforms (1) |
| GPL15379 |
Agilent-021193 Canine (V2) Gene Expression Microarray (ProbeName version) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA991983 |
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