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Sample GSM7567233 Query DataSets for GSM7567233
Status Public on Jul 11, 2023
Title MDCK Mix culture_2
Sample type RNA
 
Source name MDCK-pTR GFP-RasV12 cells co-cultured with cells at a ratio of 1:100
Organism Canis lupus familiaris
Characteristics culture condition: mixed culture
Treatment protocol MDCK-pTR GFP-RasV12 cells or a 10:1 mix culture of MDCK and MDCK-pTR GFP-RasV12 cells were cultured at a density of 1.2×107 cells on collagen-coated 10 cm dishes (CORNING). After incubation with tetracycline for 24 h at 37 ºC in a humidified incubator with 5% CO2, GFP-positive RasV12 cells was separated with an analytical flow cytometer.
Growth protocol MDCK and MDCK-pTR GFP-RasV12 cells were cultured in Dulbecco's Modified Eagle Medium, High Glucose supplemented with 10% Fetal Bovine Serum and penicillin/Streptomycin at 37 ºC in a humidified incubator with 5% CO2.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from MDCK-pTR GFP-RasV12 mCherry-β-catenin Δ131 cells using ISOGEN II (NIPPON GENE Co., Ltd).
Label Cy3
Label protocol For cRNA amplification and labeling, Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies) were used following the manufacter’s instructions.
 
Hybridization protocol 1.5 ug of Cy3-labelled cRNA was fragmented at 60°C for 30 minutes in a Agilent fragmentation buffer and was hybridized at 65°C for 17 hr using Gene Expression Hybridization Kit.
Scan protocol Microarray slides were scanned using an Agilent SureScan (G4900DA).
Description Gene expression after 24h of tetracycline treatment
Data processing The scanned images were analyzed with Feature Extraction Software (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
 
Submission date Jul 06, 2023
Last update date Jul 11, 2023
Contact name Shunsuke Kon
E-mail(s) kon44@rs.tus.ac.jp
Phone +81-4-7121-4053
Organization name Tokyo University of Science
Department Research Institute for Biomedical Sciences
Lab Division of Cancer Biology
Street address 2669 Yamazaki
City Noda-shi
State/province Chiba
ZIP/Postal code 278-0022
Country Japan
 
Platform ID GPL15379
Series (1)
GSE236658 Gene expression signatures of MDCK-pTR GFP-RasV12 cells cultured alone or mixed with MDCK cells

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
A_11_P085196 -2.947
A_11_P187033 -2.617
A_11_P166448 3.395
A_11_P163658 0.721
A_11_P198798 0.726
A_11_P0000024888 0.692
A_11_P0000011420 0.171
A_11_P177878 -0.862
A_11_P000008463 -3.113
A_11_P168333 -0.111
A_11_P0000020410 -3.120
A_11_P091711 0.091
A_11_P130416 -0.605
A_11_P167898 3.516
A_11_P213888 -4.477
A_11_P052641 -1.260
A_11_P0000040688 -4.349
A_11_P0000020760 0.054
A_11_P0000021178 2.566
A_11_P0000015207 -4.885

Total number of rows: 26529

Table truncated, full table size 558 Kbytes.




Supplementary file Size Download File type/resource
GSM7567233_mix2.txt.gz 8.8 Mb (ftp)(http) TXT
Processed data included within Sample table

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