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Series GSE19453 Query DataSets for GSE19453
Status Public on Dec 14, 2009
Title Transcriptional profiling of luteinising hormone receptor deficient mice before and after testosterone treatment
Organism Mus musculus
Experiment type Expression profiling by array
Summary Luteinising hormone (LH) is a key regulator of male fertility through its effects on testosterone secretion by Leydig cells. Mice in which the LH receptor is knocked out (LuRKO) show reduced testicular size, reduced testosterone, elevated serum LH, and a spermatogenic arrest that can be rescued by administration of testosterone. This study examines the onset of spermatogenic arrest in LuRKO males using transcriptional profiling of developing mutant and control testes. We also examine the initial stages of testosterone rescue of the phenotype, in order to identify key upstream regulators of testosterone-dependent spermatogenesis.
 
Overall design Three experimental series were obtained: (a) a developmental time course examining LuRKO and wild-type litter-mate testes at postpartum days 3, 8, 13, 19 and 27, taking birth as day 1; (b) a “rescue” time course following the testicular response to testosterone propionate treatment (2.5mg) at 2, 4, 8, 12, 24, and 48 hours post injection; (c) a second “rescue” time course examining the dose sensitivity of the androgen response at 24hrs post injection, using varying doses of testosterone propionate (vehicle only control, 250ng, 2.5µg, 25µg and 2.5mg). For each experimental sample, two biological replicates were obtained. Each individual replicate comprised pooled testis tissue from two animals.
 
Contributor(s) Ellis PJ, Griffin DK, Dunmore BJ, Bauer J, Abel MH, Affara NA
Citation(s) 20164437
Submission date Dec 14, 2009
Last update date Jan 16, 2019
Contact name Peter James Ellis
E-mail(s) p.j.i.ellis@kent.ac.uk
Phone 07808167834
Organization name University of Kent
Department School of Biosciences
Street address Stacey Building
City Canterbury
State/province Kent
ZIP/Postal code CT2 7NJ
Country United Kingdom
 
Platforms (1)
GPL6887 Illumina MouseWG-6 v2.0 expression beadchip
Samples (38)
GSM484753 LuRKO d19 testis + 2.5mg testosterone - 2hA
GSM484754 LuRKO d19 testis + 2.5mg testosterone - 2hB
GSM484755 LuRKO d19 testis + 2.5mg testosterone - 4hA
Relations
BioProject PRJNA120315

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE19453_RAW.tar 15.8 Mb (http)(custom) TAR
GSE19453_non-normalized_data.txt.gz 10.4 Mb (ftp)(http) TXT
Processed data included within Sample table
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