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| Status |
Public on Mar 18, 2021 |
| Title |
Unique human beta-cell senescence-associated secretory phenotype (SASP) reveal conserved signaling pathways and heterogeneous factors |
| Organism |
Homo sapiens |
| Experiment type |
Protein profiling by protein array
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| Summary |
The aging of pancreatic beta-cells may undermine their ability to compensate for insulin resistance, leading to the development of type 2 diabetes (T2D). Aging beta-cells acquire markers of cellular senescence and develop a senescence-associated secretory phenotype (SASP) that can lead to senescence and dysfunction of neighboring cells through paracrine actions, contributing to beta-cell failure. Herein, we defined the beta-cell SASP signature based on unbiased proteomic analysis of conditioned media of cells obtained from human senescent beta-cells. These experiments revealed that the beta-cell SASP is enriched for factors associated with inflammation, cellular stress response, and extracellular matrix remodeling across species.
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| Overall design |
After FACS-sorting, beta-Gal-positive and negative primary beta-cells were plated in 96-well plates and incubated in serum-free islet media to generate conditioned media. Conditioned media were analyzed using SomaScan at the Genomics Proteomics Core at Beth Israel Deaconess Medical Center.
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| Contributor(s) |
Midha A, Aguayo-Mazzucato C |
| Citation(s) |
33674410 |
| Submission date |
Dec 02, 2020 |
| Last update date |
Mar 18, 2021 |
| Contact name |
Hui Pan |
| E-mail(s) |
Hui.Pan@joslin.harvard.edu
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| Organization name |
Joslin Diabetes Center
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| Department |
Bioinformatics and Biostatistics Core
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| Street address |
1 Joslin Place
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| City |
Boston |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
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| Samples (27)
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| Relations |
| BioProject |
PRJNA682140 |
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